Product & ReviewsAntibodies
anti-Actin mouse monoclonal, 2G2, supernatant
Product Details
- Cat. No.
- 651132
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
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Description
Specific for skeletal and non-muscle actin isoforms (Mr 43,000). Epitope location: NVPAMYVAVLDSGVTHNVPIYHAIMRLDLA.When using antibody 2G2 in immunolocalization studies the presence of nuclear actin could be demonstrated e.g. in cultured cells after fixation with 4% paraformaldehyde. Immunostaining with cytoplasmic actin was only positive after fixation with methanol (Gonsior et al., 1999)
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Bovine, Chicken, Human, Plants, Rabbit (Lapine)
- Antigen: profilin actin complex from calf thymus
- Clone: 2G2
- Conjugate: unconjugated
- Isotype: IgM
Handling
- Quantity: 5 mL
- Storage: short term at 2-8°C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Applications
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Western Blotting (WB)