Anti-CD274 monoclonal antibody, clone MIH6 [FITC]

Rat Anti-Mouse CD274 monoclonal antibody for FC. Rat anti Mouse CD274 antibody, clone MIH6 detects mouse CD274, also known as B7-H1 and PD-1L, a single pass type I cell membrane glycoprotein, a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma. CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of na?ve T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to costimulate early T cell priming and differentiation. Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers. Flow Cytometry Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Applications：FC

• Clonality: Monoclonal
• Host: Rat
• Reactivity: Mouse
• Conjugate: FITC
• Isotype: IgG2a
• Gene: 60533

• Quantity: 100 μg
• Storage: in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

• Flow Cytometry (FC)