Anti-CD28 monoclonal antibody, clone YTH913.12 [R-PE]

Rat Anti-Human CD28 monoclonal antibody for FC. Rat anti Human CD28 antibody, clone YTH913.12 recognizes human CD28, a ~44kDa single pass type 1 trans-membrane protein expressed as a homodimer on a major subset of human T-cells, responsible for activation of these cells via interaction with the TCR. CD28 is involved in the tuning of the T-cell for activation via TCR, lowering the threshold for activation from around 8000 triggered TCRs to approximately 1500. CD28 along with CD152, also known as CTLA-4 acts as a co-receptor for the co-stimulatory molecules CD80 and CD86. CD28 offers a positive stimulatory role on ligation of CD80 and CD86 while CTLA-4 offers a negative feedback signal preventing CD28 mediated T-cell activation of CD86. Rat anti human CD28, clone YTH913.12 has been reported to recognize an epitope of CD28 expressed by NK cells, which is not recognized by other anti human CD28 clones such as 9.3 and CD28.2. Other reports however have failed to demonstrate CD28 staining on peripheral blood derived KK cells using clone YTH913.12. Flow Cytometry Use 10ul of the suggested working dilution to label 106 cells or 100ul human whole blood. Applications：FC

• Clonality: Monoclonal
• Host: Rat
• Reactivity: Human
• Conjugate: R-PE
• Isotype: IgG2b
• Gene: 940

• Quantity: 100 Tests
• Storage: Prior to reconstitution store at +4°C. Following reconstitution store at +4°C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

• Flow Cytometry (FC)