Anti-CD3D monoclonal antibody, clone MEM-57 [FITC]

Mouse Anti-Human CD3D monoclonal antibody for FC. Mouse anti Human CD3 (MEM-57) is a monoclonal antibody recognizing an extracellular epitope of the human CD3 complex containing either δ-ε or γ-ε subunit complexes . Mouse anti Human CD3 (MEM-57) recognizes a conformational CD3 epitope minimally requiring the presence of the CD3ε subunit as it will recognize murine 3D054.8 hybridoma cells tranfected with cDNA to express the human CDε subunit lacking its cytoplasmic domain. CD3 serves as a pan T cell marker, it is expressed on the surface of over 95% of circulating human peripheral T cells. CD3 is also present on ~60-80% of thymocytes and on Purkinji cells, neurons present in the cerebellar cortex. CD3 is not expressed on B cells or NK cells. The TCR complex consists of TCR heterodimers, either α/β or γ/δ expressed in a mutually exclusive manner, analagous that seen in mice on different T cell lineages. In order for the TCR heterodimer to be expressed on the cell surface it must associate with a minimal CD3 complex. The CD3 complex itself is made up of four subunits, γ, δ, ε and ζ responsible for effecitng signalling upon TCR activation, via a single or multiple, depending on the subunit, immunoreceptor tyrosine-based activation motif (ITAM) present in the CD3 subunit cytoplasmic domain. Mouse anti Human CD3 clone MEM-57 demonstrates a mitogenic effect on peripheral blood T cells and on the lymphoblastoid Jurkat cell line. Clone MEM-57 immunoprecipitates proteins of ~22kDa and more weakly ~28kDa. from peripheral blood T cell lysates. Flow Cytometry Use 10ul of the suggested working dilution to label 106 cells in 100ul or 100ul whole blood. Applications：FC

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Chimpanzee, Human
• Conjugate: FITC
• Isotype: IgG2a
• Gene: 915

• Quantity: 100 μg
• Storage: in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

• Flow Cytometry (FC)