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Mouse Anti-Human CD3E monoclonal antibody for FC. Mouse anti Human CD3 antibody, clone UCHT1 recognizes the human T-cell surface glycoprotein CD3 epsilon chain, also known as T-cell surface antigen T3/Leu-4 epsilon chain or CD3ε. CD3ε is a 207 amino acid, ~21kDa single pass type 1 transmembrane protein containing a single Ig-like and a single ITAM domain. Mouse anti Human CD3 antibody, clone UCHT1 was originally described as only binding to CD3ε when complexed with either the CD3δ or CD3γ subunits, as indicated by co-transfection immunofluorescence studies on COS cells. Mouse anti Human CD3 antibody, clone UCHT1 binds to a region in the ectodomain of human CD3ε and has been shown to bind to a discontinuous epitope near an acidic region of CD3ε opposite the dimer interface; as shown by crystallographic studies of the CD3ε/δ dimer complexed with a single chain UCHT1 antibody fragment. CD3 is expressed by all T lymphocytes and is seen in all lymphoid organs including lymph nodes and spleen. It is involved in thymocyte differentiation. Deficiency of the CD3ε chain contriutes to blocking T-cell development and presentation of a severe combined immunodeficiency phenotype. Mouse anti Human CD3 antibody, clone UCHT1 has been used successfully for the activation of human paripheral blood lymphocytes by cross linking and subsequently for CD3ε surface espression by flow cytometry. Flow Cytometry Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood. Applications：FC