Anti-CD58 monoclonal antibody, clone BRIC5 [Biotin]

Mouse Anti-Human CD58 monoclonal antibody for FC; WB. Mouse anti Human CD58 antibody, clone BRIC5 recognizes human Lymphocyte function- associated antigen 3, also known as CD58 or LFA-3. CD58 is a 250 amino acid single pass type I transmembrane glycoprotein, a member of the immunoglobulin superfamily, with a predicted molecular mass of 28.1 kDa and an apparent molecular mass of ~55-70 kDa. CD58 occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA and is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes.CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria. Mouse anti Human CD58 antibody, clone BRIC5 was produced in response to erythrocytes. The functional affinity of BRIC5 binding to erythrocytes is 4 x 108 M-1. It reacts by immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect haemagglutinin. The antigen on erythrocytes is pronase sensitive. BRIC5 inhibits T cell rosetting. Flow Cytometry Use 10ul of the suggested working dilution to label 106 cells in 100ul. Applications：FC; WB

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Human
• Conjugate: Biotin
• Isotype: IgG2a
• Gene: 965

• Quantity: 100 μg
• Storage: in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

• Flow Cytometry (FC)