Anti-CD68 monoclonal antibody, clone FA-11 [Biotin]

Rat Anti-Mouse CD68 monoclonal antibody for FC. Rat anti mouse CD68 antibody, clone FA-11 recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, which is classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain. CD68 is considered a pan macrophage marker, predominantly expressed on the intracellular lysosomes of tissue macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes. CD68 is expressed by many tumor types including some B cell lymphomas, blastic NK lymphomas, melanomas, granulocytic (myeloid) sarcomas, hairy cell leukemias, and renal, urinary and pancreatic tumors, and can be used in cancer studies to demonstrate the presence/localization of macrophages. Rat anti mouse CD68 antibody, clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical studies, using both frozen and paraffin- embedded tissues. Rat anti mouse CD68 antibody, clone FA-11 can be used in flow cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation. Flow Cytometry Use 10ul of the suggested working dilution to label 106 cells in 100ul. Applications：FC

• Clonality: Monoclonal
• Host: Rat
• Reactivity: Mouse
• Conjugate: Biotin
• Isotype: IgG2a
• Gene: 12514

• Quantity: 100 μg
• Storage: in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

• Flow Cytometry (FC)