Anti-CD80 monoclonal antibody, clone MEM-233 [R-PE]

Mouse Anti-Human CD80 monoclonal antibody for FC. Mouse anti Human CD80 antibody, clone MEM-233 recognizes human CD80, also known as B7-1, a ~60kDa type 1 trans-membrane protein expressed of macrophages, dendritic cells and activated B-cells. CD80 is a member of the immunoglobulin superfamily having an extracellular domain bearing both a single Ig-v-like domain, a single Ig-c-like domain, a transmembrane sequence and a short cytoplasmic domain. Although the predicted molecular weight for human CD80 is ~33kDa, the presence of multiple potential N-glycosylation sites results in a migration corresponding to ~60kDa. Human CD80 along with CD86 act as co-stimulatory molecules and are both ligands for CD28 and CTLA-4 involved in T cell activation and proliferation. Although CD80 binds to the same receptors as CD86 it displays quite different characteristics in its avidity and binding kinetics. Site mutagenesis studies indicate residues in both the Ig-v-like and Ig-c-like domains of CD80 are crucial for the interaction with it's receptors CTLA-4 and CD28. Mouse anti human CD80 antibody, clone MEM-233 binds to residues within the Ig-v-like domain of human CD80 as shown by domain switching studies. Functional studies using Mouse anti Human CD80, clone MEM-233 in combination with Mouse anti Human CD86, clone Bu63 suggest that clone MEM-233 is able to block binding of human CD80 with its cognate ligands CD28 and CTLA-4. Flow Cytometry Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood. Applications：FC

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Human
• Conjugate: R-PE
• Isotype: IgG1
• Gene: 941

• Quantity: 25 Tests
• Storage: Store at +4°C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

• Flow Cytometry (FC)