Product & ReviewsAntibodies

Anti-CD99 monoclonal antibody, clone DN16 [FITC]

Product Details

Cat. No.
CABT-49635MH
Type
Primary Antibody
Clonality
Monoclonal
Host
Mouse
Creative Diagnostics

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Description

Mouse Anti-Human CD99 monoclonal antibody for FC. Mouse anti human CD99 antibody, clone DN16 recognizes human CD99, also known as E2 antigen, MIC2 or 12E7. CD99 is a 185 amino acid ~32kDa single pass type I transmembrane O-glycosylated glycoprotein. Three isoforms can be producted by alternative splicing. Epitope analysis of the DN16 clone suggests the antibody recognizes a minimal peptide sequence 'LPDNENKK'; located between residues 32 and 39 towards the N-terminal region of the molecule. This sequence is present in both isoforms I and II but is largely absent from isoform 3 suggesting that the antibody will only recognize isoforms I and II. CD99 expression is notable in the testis, pancreas, bone marrow, lymph nodes and spleen. CD99 is expressed on all classes of leukocytes and tends to be highest on immature cells. Functionally CD99 has been found to be involved in cellular adhesion/aggregation and apoptosis. Flow Cytometry Use 10ul of the suggested working dilution to label 106 cells in 100ul. Applications:FC

Biological Information

  • Clonality: Monoclonal
  • Host: Mouse
  • Reactivity: Human
  • Conjugate: FITC
  • Isotype: IgG1
  • Gene: 4267

Handling

  • Quantity: 100 μg
  • Storage: in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Applications

  • Flow Cytometry (FC)
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