Anti-CTLA4 monoclonal antibody, clone BNI3 [R-PE]

Mouse Anti-Human CTLA4 monoclonal antibody for FC. Mouse anti Human CD152 antibody, clone BNI3 recognizes human CD152, also known as CTLA-4 (cytotoxic T-lymphocyte-associated antigen 4), an inhibitory receptor and negative regulator of T-cell responses. CD152 is a single pass type 1 transmembrane protein belonging to the immunoglobulin superfamily containing a single Ig-v-like domain in the extracellular region. CD152 along with CD28 binds to the co-stimulatory molecules CD80 and CD86. Mouse anti human CD152 antibody, clone BNI3 is able to block ligand binding on the Raji B-cell line and blocks binding of an alternative clone, BNI8 to CTLA-4/Ig in ELISA. Further studies indicate that clone BNI3 binds to the same epitope as classified anti CTLA-4 clones 11D4 and 10A8. The cytoplasmic domain of CD152 contains a critical tyrosine at residue 201 phosphorylated by Janus Kinase 2 which subsequently controls surface expression through regulation of CD152 interaction with AP-2. CD152 is expressed primarily as an intracellular antigen with transport to the cell surface under tight regulation of several molecules including Trim, PLD and TIRC7, CD152 also demonstrates rapid internalization once expressed at the cell surface. CD152 plays a significant role in maintaining tolerance to self antigens and defects in CD152 presentation and expression has been implicated in a number of autoimmune diseases. Flow Cytometry Use 20ul of the suggested working dilution to label 5x105 cells in 100ul. Applications：FC

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Human
• Conjugate: R-PE
• Isotype: IgG2a
• Gene: 1493

• Quantity: 100 Tests
• Storage: Store at +4°C. DO NOT FREEZE This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

• Flow Cytometry (FC)