Product & ReviewsAntibodies
Anti-F4/80 antibody [CI:A3-1]
Product Details
- Cat. No.
- ab6640
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Rat
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Description
Rat monoclonal [CI:A3-1] to F4/80
Biological Information
- Clonality: Monoclonal
- Host: Rat
- Reactivity: Mouse
- Isotype: IgG2b
Handling
- Quantity: 100 µg
- Storage: Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Buffer: pH: 7.60, Preservative: 0.01% Thimerosal (merthiolate), Constituents: 0.164% Sodium phosphate, 1.45% Sodium chloride, 1% BSA
- Specificity: 5
Applications
- Flow Cytometry (FC)
- Immunocytochemistry (ICC)
- Immunofluorescence (IF)
- Immunohistochemistry (Frozen Sections) (IHC (Fr))
- Immunohistochemistry (PFA Perfusion Fixed Frozen Sections) (IHC (FoFr))
- Immunoprecipitation (IP)
- Radioimmunoassay (RIA)
- Western Blotting (WB)
Application Area:
Immunochistochemistry
We used Anti-F4/80 antibody [CI:A3-1] (ab6640) for staining macrophages in aortic root plaque. The samples were fixed in PFA and then frozen in OC. Despite that samples were definitely over fixed, the antibody worked very good, quite sensitive, the staining was reproducible with quite straight forward protocol.
Review Date: 5 Nov 2018 | Abcam plc