Anti-His tag antibody

Chicken anti-His tag polyclonal antibody for WB, IP. Polyclonal Antibody to 6X-Histidine. Chickens were immunized with the 6X-Histidine peptide conjugated to keyhole limpet hemocyanin protein and emulsified in Freund's adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using 6X-Histidine peptide conjugated to an agarose matrix. To make up the final mixture, the IgY fraction was spiked with affinity purified antibody, mixed with glycerol and thimerosal, and filter-sterilized.Polyclonal Antibody to 6X-Histidine. Chickens were immunized with the 6X-Histidine peptide conjugated to keyhole limpet hemocyanin protein and emulsified in Freund's adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using 6X-Histidine peptide conjugated to an agarose matrix. To make up the final mixture, the IgY fraction was spiked with affinity purified antibody, mixed with glycerol and thimerosal, and filter-sterilized. Applications：WB

• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated

• Quantity: 100 μl
• Storage: Store at -20oC in the dark. Under these conditions, these antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies at –80oC unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.
• Buffer: Sodium phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS," pH 7.2) (50% by volume) with glycerol (50% by volume) as an anti-freezing agent and thimerosal (0.01%, w/v) as a preservative.

• Flow Cytometry (FC)