Anti-Human EG-VEGF (Clone 188608)

Endocrine Gland-Derived Vascular Endothelial Growth Factor (EG-VEGF) or Prokineticin-1 (PK-1) is a novel secreted protein expressed in various tissues including steroidogenic glands. Although structurally non-related to VEGF, EG-VEGF/PK-1 was shown to promote angiogenesis acting via two G-protein coupled receptors (PK-R1 and PK-R2). 1 EG-VEGF is mitogen specific for the endothelium of steroidogenic glands. It induces proliferation, migration and fenestration in capillary endothelial cells derived from endocrine glands. EG-VEGF mRNA expression is localized to the steroid hormone-producing cells, i.e. Leydig cells of the testis and the specialized stroma in the ovary. 2 EG-VEGF increased the phosphorylation of Akt in a phosphatidylinositol 3-kinase-dependent manner and is a survival factor for ACE cells. EG-VEGF, possibly through binding to a G-protein coupled receptor, results in the activation of MAPK p44/42 and phosphatidylinositol 3-kinase signaling pathways, leading to proliferation, migration, and survival of responsive endothelial cells. 3

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Human
• Antigen: Human EG-VEGF is a secreted angiogenetic mitogen growth factor expressed in the steroidogenic glands, ovary, testis, adrenal and placenta.
• Clone: 188608
• Conjugate: Purified No Carrier Protein
• Isotype: IgG1

• Quantity: 500 µg
• Storage: The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

• Immunohistochemistry (IHC)
• In Situ Hybridization (ISH)
• Mass Cytometry (CyTOF)
• Western Blotting (WB)