Anti-Human HLA-A, B, C (MHC Class I) - Purified <i>In vivo </i>GOLD™ Functional Grade

HLA antibody, clone W6/32, recognizes the major histocompatibility complex (MHC) class I molecules human leukocyte antigen (HLA)-A, HLA-B, and HLA-C. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. MHC class I plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11.

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Human
• Antigen: HLA-A, -B, and -C are ubiquitously expressed on nucleated cells.
• Clone: W6/32
• Conjugate: GOLD, Low Endotoxin Functional Formulation
• Isotype: IgG2a κ
• Gene: 3105

• Quantity: 25 mg
• Storage: This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

• Blocking (BA)
• Immunohistochemistry (IHC)
• Immunoprecipitation (IP)
• In Situ Hybridization (ISH)
• Western Blotting (WB)