Anti-Human IL-5 Rα - Biotin

The functional IL-5 receptor is a heteromeric complex consisting of an alpha and beta subunit.1 The IL-5Rα chain is exclusively expressed by eosinophils, some basophils and murine B1 cells or B cell precursors.2 Like many other cytokine receptors, alternative splicing of the α-chain gene results in expression of either a membrane bound or soluble form of the α-chain. The soluble form does not lead to signal transduction and therefore has an antagonistic effect on IL-5 signaling. Both monomeric forms of IL-5Rα are low affinity receptors, while dimerization with the β-chain produces a high affinity receptor.3 In either case, the α-chain exclusively binds IL-5 and the intra-cellular portion of IL-5Rα is associated with Janus kinase (JAK) 2, a protein tyrosine-kinase essential in IL-5 signal transduction.4,5 Soluble IL-5Ralpha expression pattern in an eosinophil-associated disease such as nasal polyposis (NP).6

• Clonality: Polyclonal
• Host: Goat
• Reactivity: Human
• Conjugate: Biotin
• Gene: 3568

• Quantity: 50 µg
• Storage: The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

• ELISA (ELISA)
• In Situ Hybridization (ISH)
• Western Blotting (WB)