Anti-IL2RA monoclonal antibody, clone 9.14 [FITC]

Mouse Anti-Sheep IL2RA monoclonal antibody for FC. Mouse anti sheep CD25, clone 9.14, is specific for the ovine homologue of human CD25, also known as Interleukin-2 receptor alpha chain (IL-2Rα), a single pass type I membrane protein expressed by activated ovine T-cells. Ovine Interleukin-2 is a cytokine involved in the proliferation, growth and differentiation of T-cells, B-cells and NK cells and its receptor is composed of 3 subunits, an α chain (CD25), a β chain (CD122) and a γ chain (CD132). A non-covalent association of the α and β subunits is required to form the high affinity receptor for IL-2. Immunoprecipitation studies have demonstrated that clone 9.14 precipitates a band of approximately 47kDa under reducing conditions, which is that expected of the mature protein due to high glycosylation, and is consistent with the observed molecular weight of IL-2Rα in other species such as humans. Research has demonstrated that antibodies to CD4, FoxP3 and CD25 may be used in studies of T regulatory cells (Tregs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity. Flow Cytometry Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul. Applications：FC

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Sheep (Ovine)
• Conjugate: FITC
• Isotype: IgG1
• Gene: 443435

• Quantity: 100 μg
• Storage: in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

• Flow Cytometry (FC)