Anti-IL4 monoclonal antibody, clone CC314 [Biotin]

Mouse Anti-Bovine IL4 monoclonal antibody for ELISA; FC. Mouse anti bovine interleukin-4 antibody, clone CC314 recognizes bovine intetleukin-4 (IL-4), also known as B-cell stimulatory factor 1. IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses. Bovine interleukin-4 is a 15kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6kDa active cytokine, the apparent Mw may be altered by glycosylation. IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses. Mouse anti Bovine interleukin-4, clone CC314 has been used successfully as a detection antibody in a sandwich ELISA with Clone CC313 as a coating reagent both with bovine and ovine samples. Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples. Flow Cytometry Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ELISA This biotin conjugate may be used as detection reagent in sandwich ELISA assays for bovine IL-4 with MCA2371 as capture reagent, and recombinant bovine IL-4 (PBP006) as a standard. Applications：ELISA; FC

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Bovine, Goat, Sheep (Ovine)
• Conjugate: Biotin
• Isotype: IgG2b
• Gene: 280824

• Quantity: 250 μg
• Storage: in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

• Flow Cytometry (FC)