anti-Keratin K13, Ks13.1 + positive western blot control

anti-Keratin K13 antibody:Ks 13.1 represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin. Polypeptide reacting: Mr 54 000 polypeptide human keratin K13 (formerly designated cytokeratin13; with minor affinity to keratins K14, Mr 50 000, and K16, Mr 48 000 and also to K25, formerly designated K25irs1 from inner root sheath of hair follicle).Tumors specifically detected: several squamous cell carcinomas, e.g. cervix carcinoma; transitional cell carcinoma of the bladder; craniopharyngiomaReactivity on cultured cell lines: Cell lines from squamous cell CA, e.g. A-431 from epidermoid CA of vulva; RT 112, RT-4 of urinary bladder CAPositive western blot control: Whole Cell Lysate from MCF7 human breast adenocarcinoma cell line.MCF7 whole cell lysate was prepared by homogenization in PBS containing Pefablock.Protein concentration was determined using Bradford assay.Set content: Cat. No. 61007, anti-Keratin K13 mouse monoclonal, Ks13.1, lyophilized, purifiedCat. No. 64010, Positive western blot control: anti-Keratin K13 antibody

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Bovine, Human, Rat
• Antigen: Keratin K13 of Mr 54 000 purified from human esophagus
• Clone: Ks13.1
• Conjugate: unconjugated
• Isotype: IgG1

• Quantity: 50 µg anti-Keratin K13 + 50 µg western blot control
• Storage: antibody: short term at 2-8°C; long term in aliquots at -20°C; western blot control: lyophilized at 2-8°C, reconstituted at -20°C; avoid freeze/ thaw cycles for both components

• Immunocytochemistry (ICC)
• Immunohistochemistry (IHC)
• Western Blotting (WB)