anti-Keratin K18, Ks18.04 + positive western blot control
Product Details
- Cat. No.
- 61028-WBC
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
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Description
anti-Keratin K18 antibody:Ks18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.Tested cultured cell lines: MCF-7Positive western blot control: Whole Cell Lysate from MCF7 human breast adenocarcinoma cell line.MCF7 whole cell lysate was prepared by homogenization in PBS containing Pefablock.Protein concentration was determined using Bradford assay.Set content: Cat. No. 61028, anti-Keratin K18 mouse monoclonal, Ks18.04, lyophilized, purifiedCat. No. 64007, Positive western blot control: anti-Keratin K18 antibody
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Bovine, Dog (Canine), Hamster, Human, Mouse, Pig (Swine), Rat, Sheep (Ovine), Trout, Zebrafish
- Antigen: human keratin K18 from HeLa cytoskeletal preparation
- Clone: Ks18.04
- Conjugate: unconjugated
- Isotype: IgG1
Handling
- Quantity: 50 µg anti-Keratin K18 + 50 µg western blot control
- Storage: antibody: short term at 2-8°C; long term in aliquots at -20°C; western blot control: lyophilized at 2-8°C, reconstituted at -20°C; avoid freeze/ thaw cycles for both components
Applications
- ELISA (ELISA)
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Western Blotting (WB)