Product & ReviewsAntibodies
anti-Keratin K8/K18 mouse monoclonal, NCL-5D3, supernatant
Product Details
- Cat. No.
- 10502
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
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Description
The antibody recognizes all simple epithelia including glandular epithelia. It does not react with stratified squamous epithelium. Useful for the differentiation between carcinomas and lymphomas. In immunoblotting (Western) NCL 5D3 is directed against the the 52.2 kD keratin K8 (originally reported also to 45 kD keratin K18; formerly also designated cytokeratins according to the Moll-catalogue). Epitope has been mapped to aa 353-367 on alpha helical rod domain of keratin K8 (Waseem et al
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Human
- Antigen: isolated from human breast cancer carcinoma cell line MCF-7
- Clone: NCL-5D3
- Conjugate: unconjugated
- Isotype: IgG1
Handling
- Quantity: 1 mL
- Storage: short term at 2-8°C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Applications
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Western Blotting (WB)