anti-Keratin K8/K18 + positive western blot control
Product Details
- Cat. No.
- GP11-WBC
- Type
- Primary Antibody
- Clonality
- Polyclonal
- Host
- Guinea Pig
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Description
anti-Keratin K8/K18 antibody:eactive polypeptide (specificity): Mr 52 500 and 45 500; Keratins K8 and K18 (formerly also designated cytokeratins 8 and 18) of simple type epithelium. Tumors Specifically Detected: All adenocarcinoma tested, undifferentiated carcinoma, hepatocellular carcinoma, cervix carcinomaPositive western blot control: Whole Cell Lysate from MCF7 human breast adenocarcinoma cell line.MCF7 whole cell lysate was prepared by homogenization in PBS containing Pefablock. Protein concentration was determined using Bradford assay.Set content: Cat. No. GP11, anti-Keratin K8/K18 guinea pig polyclonal, serumCat. No. 64004, Positive western blot control: anti-Keratin K8/K18 antibody
Biological Information
- Clonality: Polyclonal
- Host: Guinea Pig
- Reactivity: Bovine, Chicken, Human, Mouse, Rat, Syrian Hamster
- Antigen: Keratin K8/K18 filaments, reconstituted from purified bovine keratins K8 and K18
- Conjugate: unconjugated
Handling
- Quantity: 100 µl anti-Keratin K8/K18 + 50 µg western blot control
- Storage: antibody: short term at 2-8°C; long term in aliquots at -20°C; western blot control: lyophilized at 2-8°C, reconstituted at -20°C; avoid freeze/ thaw cycles for both components
Applications
- Immunohistochemistry (IHC)
- Western Blotting (WB)