Product & ReviewsAntibodies

anti-Keratin K8, Ks8.7 + positive western blot control

Product Details

Cat. No.
61038-WBC
Type
Primary Antibody
Clonality
Monoclonal
Host
Mouse
PROGEN

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Description

anti-Keratin K8 antibody:Ks 8.7 represents an excellent marker to discriminate simple epithelia from those of different origin.Polypeptide reacting: Mr 52 500 polypeptide (keratin K8; formerly also designated cytokeratin 8) of human epithelia.Reactivity on cultured cell lines: MCF-7Tumors specifically detected: All adenocarcinoma tested; undifferentiated carcinoma; cervix carcinoma; hepatocellular carcinomaPositive western blot control: Whole Cell Lysate from MCF7 human breast adenocarcinoma cell line.MCF7 whole cell lysate was prepared by homogenization in PBS containing Pefablock.Protein concentration was determined using Bradford assay.Set content: Cat. No. 61038, anti-Keratin K8 mouse monoclonal, Ks8.7, lyophilized, purifiedCat. No. 64009, Positive western blot control: anti-Keratin K8 antibody

Biological Information

  • Clonality: Monoclonal
  • Host: Mouse
  • Reactivity: Hamster, Human
  • Antigen: cytoskeletal proteins from cultured HeLa cells
  • Clone: Ks8.7
  • Conjugate: unconjugated
  • Isotype: IgG1

Handling

  • Quantity: 50 µg anti-Keratin K8 + 50 µg western blot control
  • Storage: antibody: short term at 2-8°C; long term in aliquots at -20°C; western blot control: lyophilized at 2-8°C, reconstituted at -20°C; avoid freeze/ thaw cycles for both components

Applications

  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Western Blotting (WB)
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