Anti-Mouse CD40 (Clone FGK45) - Purified <i>In vivo </i>PLATINUM™ Functional Grade
Product Details
- Cat. No.
- F1195- 50 mg
- Clonality
- Monoclonal
- Host
- Rat
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Description
CD40, also known as TNFSF5, is a type I transmembrane protein and member of the TNF receptor family. The binding of CD40L (CD154) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects (1). CD40 is expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells (2). Signaling though CD40 plays an important role in the proliferation and differentiation of B cells and is critical for immunoglobulin (Ig) class switching (3). The membrane-anchored CD40L is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. Some of the early intracellular signaling by the CD4-CD40L system includes the association of the CD40 with TRAFs and the activation of various kinases (4). Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of CD4-CD40L is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
Biological Information
- Clonality: Monoclonal
- Host: Rat
- Reactivity: Mouse
- Antigen: CD40 is widely expressed on antigen-presenting cells such as dendritic cells, B cells, macrophages, and monocytes, in addition to non-immune endothelial cells, basal epithelial cells, and a variety of tumors.
- Clone: FGK45
- Conjugate: Low Endotoxin Functional Formulation, PLATINUM
- Isotype: IgG2a
- Gene: 21947
Handling
- Quantity: 50 mg
- Storage: Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Applications
- Blocking (BA)
- Depletion (Dep)
- Immunoprecipitation (IP)
- In Situ Hybridization (ISH)
- Mass Cytometry (CyTOF)
- Western Blotting (WB)