Product & ReviewsAntibodies

Anti-Mouse H-2K<sup>d</sup> (MHC Class I) - Purified <i>In vivo </i>GOLD™ Functional Grade

Product Details

Cat. No.
H146
Clonality
Monoclonal
Host
Mouse
Leinco Technologies

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Description

H-2Kd antibody, clone A4C8.1-Do9, recognizes the major histocompatibility complex (MHC) class I H-2K haplotype d (H-2Kd). MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. H-2Kd plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11.

Biological Information

  • Clonality: Monoclonal
  • Host: Mouse
  • Reactivity: Mouse
  • Antigen: H-2Kd is ubiquitously expressed on nucleated cells from mice of the H-2Kd haplotype, including BALB/c.
  • Clone: A4C8.1-Do9
  • Conjugate: GOLD, Low Endotoxin Functional Formulation
  • Isotype: IgG1 κ

Handling

  • Quantity: 1.0 mg, 100 mg, 25 mg, 5.0 mg, 50 mg
  • Storage: Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Applications

  • Immunohistochemistry (IHC)
  • Western Blotting (WB)
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