Product & ReviewsAntibodies

anti-p21/ WAF1 mouse monoclonal, WA-1, purified

Product Details

Cat. No.
691725
Type
Primary Antibody
Clonality
Monoclonal
Host
Mouse
PROGEN

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Description

WA-1 reacts with human and other mammalian p21, a tumor suppressor protein, belonging to the CDI family. The intracellular protein p21 is a 21 kDa protein, also known as wild-type p53-activated fragment 1 (WAF1). It is an inhibitor of cyclin-dependent kinases (Cdks) and of proliferating-cell nuclear antigen (PCNA). It is induced by wild type p53, but not by mutated p53, by mezerein (anti-leukemic compound) and by interferon-ß. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma and thyroid carcinoma).Positive control: MCF7 cells, UV treated fibroblasts, HeLa cells, skin, colon, or breast carcinoma.

Biological Information

  • Clonality: Monoclonal
  • Host: Mouse
  • Reactivity: Human, Monkey, Mouse, Rat
  • Antigen: human p21 protein
  • Clone: WA-1
  • Conjugate: unconjugated
  • Isotype: IgG1 kappa

Handling

  • Quantity: 1 mL (100 µg/ml)
  • Storage: store at 2-8°C

Applications

  • ELISA (ELISA)
  • Flow Cytometry (FC)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
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