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Anti-SELL monoclonal antibody, clone CC32 [FITC]

Product Details

Cat. No.
CABT-46537MB
Type
Primary Antibody
Clonality
Monoclonal
Host
Mouse
Creative Diagnostics

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Description

Mouse Anti-Bovine SELL monoclonal antibody for FC. Mouse anti Bovine CD62L antibody, clone CC32 recognizes bovine L-selectin, also known as CD62L, Leukocyte-endothelial cell adhesion molecule 1, LECAM-1 or Lymph node homing receptor. Bovine CD62L is a 370 amino acid ~90kDa, single pass type I transmembrane glycoprotein bearing a single C-type lectin domain, an EGF-like domain and two Sushi domains (UniProt: P98131). Immunoprecipitation of peripheral blood mononuclear cell lysates with Mouse anti Bovine CD62L antibody, clone CC32 reveals a molecule of ~90kDa when run on polyacrylamide gels under reducing conditions, slightly larger than the murine and human CD62L homologues. Bovine CD62L is expressed on subpopulations of T-lymphocytes expressing CD2, CD4 and CD8. WC1 +ve positive γ/δ T cells also express CD62L as do a subpopulation of WC3 all peripheral blood monocytes. lymphocytes andMouse anti Bovine CD62L antibody, clone CC32 has also been used successfully for the identification of CD62L on ovine peripheral blood cells by flow cytometry.Flow Cytometry Use 10ul of the suggested working dilution to label 106 cells in 100ul. Applications:FC

Biological Information

  • Clonality: Monoclonal
  • Host: Mouse
  • Reactivity: Bovine
  • Conjugate: FITC
  • Isotype: IgG1
  • Gene: 281485

Handling

  • Quantity: 100 μg
  • Storage: in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Applications

  • Flow Cytometry (FC)
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