Anti-SIRPA monoclonal antibody, clone 15-414 [R-PE]

Mouse Anti-Human SIRPA monoclonal antibody for FC. Mouse anti Human CD172a antibody, clone 15-414 recognizes human CD172a, also known as signal-regulatory protein alpha, a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells. CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase- coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs. Flow Cytometry Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Applications：FC

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Human
• Conjugate: R-PE
• Isotype: IgG2a
• Gene: 140885

• Quantity: 100 Tests
• Storage: Prior to reconstitution store at +4°C. After reconstitution store at +4°C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

• Flow Cytometry (FC)