anti-Thomsen Friedenreich Antigen mouse monoclonal, A78-G/A7, supernatant
Product Details
- Cat. No.
- 65176
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
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Description
The antigen / epitope is mainly found on the cell membrane. A78-G/A7 binds to CD176 cell surface antigen / asialoglyco-phorin but not to glycophorin. It agglutinates neuraminidase-treated but not untreated human erythrocytes. The epitope is sensitive to periodate oxidation. Positive reactions are found for primary breast cancer and colorectal cancer and metastases derived therefrom. Normal colonic adjacent tissue is negative, whereas breast epithelium may react, esp. luminal membranes. In immunocytochemis-try positive staining is also found with acute myelogeneous leukemia cells (HL-60, KG-1).In immunoblotting a major band of 155 kDa is detectable. Specific binding with both anomers of TF as well as with the TFß-related asialo-GM1.
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Human
- Antigen: human asialoglycophorin A
- Clone: A78-G/A7
- Conjugate: unconjugated
- Isotype: IgM
Handling
- Quantity: 1 mL
- Storage: short term at 2-8°C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Applications
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blotting (WB)