anti-Vimentin mouse monoclonal, VIM 3B4 + anti-mouse IgG HRP set

anti-Vimentin antibody:The antibody is highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. VIM 3B4 has turned out to be the most avid mab to vimentin. Polypeptide reacting: Mr 57 000 intermediate filament protein (vimentin) of mesenchymal cells. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma.The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).Bohn W, Wiegers W, Beuttenmüller M, Traub P: Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp Cell Res 201: 1-7 (1992).Rogers KR, Eckelt A, Nimmrich V, Janssen K-P, Schliwa M, Herrmann H, Franke WW: Truncation mutagenesis of the non-alpha-helical carboxyterminal tail domain of vimentin reveals contributions to cellular localization but not to filament assembly. Eur J Cell Biol 66: 136-150 (1995).anti-mouse IgG goat polyclonal, HRP conjugate:Whole mouse IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.Set content: Cat. No. 61013, anti-Vimentin mouse monoclonal, VIM 3B4, lyophilized, purifiedCat. No. 90002, anti-mouse IgG goat polyclonal, HRP conjugate

• Clonality: Monoclonal
• Reactivity: See individual antibody datasheet
• Antigen: see individual antibody datasheet for information about specific immunogens

• Quantity: 50 µg anti-Vimentin + 100 µg anti-mouse IgG HRP
• Storage: lyophilized at 2-8°C, reconstituted at -20°C (avoid freeze and thaw cycles)

• Immunohistochemistry (IHC)
• Western Blotting (WB)