Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis using the FITC conjugate of NB600-305. Detection of Lane 1 shows 80 ug and lane 2 shows 20 ug loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. Blots were blocked overnight at 4C with blocking buffer for Fluorescent Western blotting conjugated Gt-a-anti-Rabbit IgG was used for detection. Molecular weight estimation was made by comparison to a prestained MW marker (center).in lane M.