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Biotinylated Anti-Avidin has been widely used as an amplifying reagent in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. The capability of binding avidin via either biotin binding sites or through antigen binding sites, makes this biotinylated antibody unique. Our antibodies to avidin are produced in goats using our highly purified avidin and isolated by affinity chromatography. Anti-Avidin does not bind streptavidin and Anti-Streptavidin does not recognize avidin. These antibodies provide opportunities to significantly amplify signals in many applications.