BRAF (1H12) Antibody

"BRAF (v-raf murine sarcoma viral oncogene homolog B1) is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428 and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region, the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma, melanocytic nevi, primary cutaneous melanomas and colorectal cancers. "

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Human, Mouse
• Antigen: "Purified recombinant fragment of human BRAF expressed in E. coli. "
• Clone: 1H12
• Isotype: Mouse IgG1
• Gene: P11345

• Quantity: 0.1 ml
• Storage: Store at 4°C for short term use only. Store at -20°C for storage over 1 month. Product is guaranteed 6 months from the date of shipment.

• ELISA (ELISA)
• Immunofluorescence (IF)
• Immunohistochemistry (IHC)
• Western Blotting (WB)