BV421 Mouse Anti-Human CD31 Clone WM59
Product Details
- Cat. No.
- 564089
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
Description
The WM59 monoclonal antibody specifically binds to platelet endothelial cell adhesion molecule-1, (PECAM-1, PECAM1), which is also known as GPIIA', or EndoCAM. CD31 is a 130 kDa type I transmembrane glycoprotein that belongs to the Ig gene superfamily. CD31 has wide tissue distribution and is expressed on platelets, monocytes, granulocytes, NK cells, T cell subsets, and in high amounts on endothelial cells. CD31 functions as a vascular endothelial cell adhesion molecule and is involved in the transendothelial migration of leucocytes in inflammatory responses. It might be involved in thrombosis, angiogenesis, and wound healing. The WM59 appears to recognize an epitope proximal to extracellular domain 2 of CD31.Clone WM59 also cross-reacts with peripheral blood platelets and leukocytes of baboon, and both rhesus and cynomolgus macaque monkeys. The staining intensity of WM59+ platelets is similiar to that observed with peripheral blood platelets from normal human donors. Lymphocytes, monocytes, and granulocytes react less with WM59 than normal human leukocytes.The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Baboon, Cynomolgus Monkey, Human, Rhesus
- Isotype: IgG1, k
- Gene: 5175
Handling
- Quantity: 50 Tests
- Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
- Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Specificity: 4
Applications
- Flow Cytometry (FC)
Flow cytometry
We used this antibody to analyze cells isolated from kidney tissue and the cell lines Caki-1 as well as ECRF24. The specificity and signal intensity measured on a BD Fortessa Cytometer was significantly better compared to antibodies from other providers. In highly vascularized tissue (kidney) we were able to obtain sufficient signal intensity at a 1:1000 dilution of the antibody.
Review Date: 14 May 2021 | BD Biosciences
Flow Cytometry
The efficacy and specificity is very good, signal intensity is sufficient to analyze a pool of 2 endothelial cell lines at the same time. Separation is good.
Review Date: 6 Feb 2019 | BD Biosciences