CD45-PE, Immu19.2, 100 Tests, RUO
Product Details
- Cat. No.
- IM1833
- Type
- Flow Cytometry Reagents
- Clonality
- Monoclonal
- Host
- Mouse
The supplier does not provide quotations for this antibody through SelectScience. You can search for similar antibodies in our Antibody Directory.
Description
The CD45 molecules are single chain integral membrane proteins comprising at least five isoforms ranging from 180 to 220 kDa. They are generated by alternative splicing combinations of three exons (A, B, and C) of the genomic sequence. The non-restricted CD45, Leucocyte Common Antigen (LCA) consists of an extracellular sequence, proximal to the membrane, which is common to all CD45 isoforms. All the monoclonal antibodies that belong to the CD45 cluster react with this part of the antigen and are able to recognize all CD45 isoforms. These isoforms have extracytoplasmic sequences ranging from 391 to 552 amino acids long with numerous N-linked carbohydrate attachment sites. The cytoplasmic portion contains two phospho-tyrosine-phosphatase domains. The non-restricted CD45 epitope is present on the surface of all human leucocytes, lymphocytes, eosinophils monocytes, basophils and neutrophils by order of decreasing level of expression. CD45 is a major component of the lymphocyte membrane. It is absent from erythrocytes and platelets. It is lost during maturation of erythroid cells in the bone marrow. CD45 antibodies react with leucocyte progenitors in bone marrow.
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Human
- Antigen: CD45
- Clone: Immu19.2
- Conjugate: PE
- Isotype: IgG1
Handling
- Quantity: 100 tests
- Specificity: 5
Applications
- Flow Cytometry (FC)
Performs reproducibly and cost effective.
Flow cytometery
The antibody was easy to optimize and essentially we followed the recommended dilutions for flow cytometery and it worked on our first try. The 100test product is very economical as we are performing studies on multiple patients and timepoints per patient. We have also tested this product in a panel with other adaptive immune antibodies from BD and it is working very well. We would highly recommend BD Antibodies for flow cytometery. We do not use any other company product for this work.
Review Date: 13 Feb 2023 | Beckman Coulter Life Sciences