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CD9 (p24 antigen) is a single transmembrane polypeptide of 24 kDa related to the tetraspanin (TM4) family. Like other tetraspanins (e.g. CD63, CD81, CD82, CD37, CD53, among the 20 known members), CD9 structure is composed of 4 transmembrane domains, with intracellular N and C termini. First discovered on a lymphoblastic cell line of pre-B phenotype, CD9 was then found on platelets and within their α-granules, on monocytes, pre-B cells, eosinophils, basophils and activated T cells. The CD9 molecule associates with other surface proteins such as the α6/β4 integrin (CD49f/CD104 molecule) and HLA-DR, suggesting a role in adhesion, signal transduction and cell motility.*Alexa Fluor and Pacific Blue are registered trademarks of Molecular Probes, Inc.