ChIPAb+ Acetyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set
Product Details
- Cat. No.
- 17-683
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
Description
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Acetyl-Histone H3 (Lys27) set includes the Anti-acetyl-Histone H3 (Lys27) antibody, a negative control antibody (purified Mouse IgG), and qPCR primers which amplify a 178 bp region within the promoter of the human RPL10 gene. The acetyl-histone H3 (Lys27) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of acetyl-histone H3 (Lys27)-associated chromatin.
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Human, Vertebrate
- Isotype: IgG
Handling
- Storage: Stable for 1 year at -20°C from date of receipt. Aliquot upon thawing, avoid freeze thaw cycles.
- Specificity: 5
Applications
- Chromatin Immunoprecipitation (ChIP)
- Western Blotting (WB)
ChIP and ChIP-seq
I always achieve reproducible results for ChIP experiments with this antibody. It is reliable, and easy to use. It contains primer set as negative control and positive control.
Review Date: 4 Dec 2018 | Merck KGaA, Darmstadt, Germany