Product & ReviewsAntibodies

Collagen Type I (5D8) Antibody

Product Details

Cat. No.
250274
Type
Primary Antibody
Clonality
Monoclonal
Host
Mouse
Abbiotec

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Description

Type I collagen is present in connective tissue and bone where it makes up about 90% of the organic matrix. Defects in one or both alleles of the type I collagen gene causes "brittle bone disease" (osteogenesis imperfecta). Patients suffering from such defects have low levels of fully functional type I collagen resulting in fragile bones. This antibody is highly specific for native type I collagen and shows no evidence of cross-reactivity with type II, III, V and VI collagens in inhibition ELISA and immunoblotting, and no evidence of cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). The antibody reacts with human, calf, sheep, pig, dog and goat type I collagens in ELISA and immunoblotting, but does not react with mouse, guinea pig, cat, rabbit, squirrel, horse, kangaroo or chicken type I collagen. The antibody has a Ka of approximately 2.5 x 106 M-1 for human type I collagen.

Biological Information

  • Clonality: Monoclonal
  • Host: Mouse
  • Reactivity: Human
  • Antigen: Pepsin-solubilized, salt-fractionated type I collagen from human placenta
  • Clone: 5D8
  • Isotype: Mouse IgG1/k
  • Gene: P02452

Handling

  • Quantity: 0.2 mg
  • Storage: Store at 4°C. Product is guaranteed 6 months from the date of shipment.

Applications

  • ELISA (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blotting (WB)
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