Western Blot. Positive WB detected in: U87 whole cell lysate, SY5Y whole cell lysate, PC-3 whole cell lysate, U251 whole cell lysate, K562 whole cell lysate, Mouse brain tissue, Rat brain tissue . All lanes: SDCBP antibody at 1:2000. Secondary. Goat polyclonal to mouse IgG at 1/50000 dilution. Predicted band size: 32 kDa. Observed band size: 35 KDa. Exposure time:5min