Western Blot. Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate. All lanes TUBB antibody at 1:5000. Secondary. Goat polyclonal to mouse IgG at 1/50000 dilution. Predicted band size: 55 KDa. Observed band size: 55 KDa. Exposure time:5s