Western Blot. Positive WB detected in: NFE2L2 antibody at 1:1000. Lane 1: Hela whole cell lysate. Lane 2: THP-1 whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lane 5: RAW264.7 whole cell lysate. Lane 6: K562 whole cell lysate. Secondary. Goat polyclonal to Mouse IgG at 1/20000 dilution. Predicted band size: 68 KDa. Observed band size: 68-100 KDa. Exposure time: 1min