Western blot. All lanes: Probable ATP-dependent RNA helicase DDX5 antibody at 3ug/ml. Lane 1: NIH/3T3 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 70, 61 kDa. Observed band size: 70 kDa.