Western blot. All lanes: HCCS antibody at 2.05µg/ml. Lane 1: Mouse heart tissue. Lane 2: Hela whole cell lysate. Lane 3: RAW264.7 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 31 kDa. Observed band size: 31 kDa