Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, HepG2 whole cell lysate. All lanes: HIST1H1C antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/40000 dilution. Predicted band size: 22 kDa. Observed band size: 22 kDa