Western Blot. Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate (treated by 30mM sodium crotonylate for 4h). All lanes: HIST1H3A antibody at 1.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 16 kDa. Observed band size: 16 kDa