Western Blot. Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, U251 whole cell lysate, Mouse lung tissue. All lanes: Pcsk1 antibody at 8ug/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 85 kDa. Observed band size: 85 kDa.