Western blot. All lanes: Toll/interleukin-1 receptor domain-containing adapter protein antibody at 2ug/ml. Lane 1: K562 whole cell lysate. Lane 2: Mouse kidney tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 24, 26, 28 kDa. Observed band size: 24 kDa.