Western blot. All lanes: TMED10 antibody at 4.88µg/ml. Lane 1: Hela whole cell lysate. Lane 2: Mouse liver tissue. Lane 3: Mouse lung tissue. Lane 4: 293T whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: U87 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 25 kDa. Observed band size: 25 kDa