Western Blot. Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A431 whole cell lysate. All lanes: ENO1 antibody at 2.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 48, 37 kDa. Observed band size: 48 kDa