Western blot. All lanes: AQP1 antibody at 16µg/ml. Lane 1: Rat heart tissue. Lane 2: Mouse skeletal muscle tissue. Lane 3: A549 whole cell lysate. Lane 4: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 29, 20, 24, 17 kDa. Observed band size: 29 kDa