Western Blot. Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: RUFY3 antibody at 3.71µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53, 56, 71, 65 kDa. Observed band size: 53 kDa